Protein Engineering

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Protein engineering is the process of developing useful or valuable proteins. There are two general strategies for protein engineering: rational protein design and directed evolution. These methods are not mutually exclusive; researchers will often apply both. In the future, more detailed knowledge of protein structure and function, and advances in high-throughput screening, may greatly expand the abilities of protein engineering. Eventually, even unnatural amino acids may be included, via newer methods, such as expanded genetic code, that allow encoding novel amino acids in genetic code.

Engineered proteins can be used in :- Detergent industry applications (proteases), Biosensors , Medical applications, Biopolymer production applications , Nano biotechnology applications , Applications with redox proteins and enzymes

Methods by which we can achieve engineered proteins

  • Site-directed mutagenesis
  • DNA shuffling
  • Molecular dynamics
  • Homology modelling
  • De novo enzyme engineering

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