Synthetic Biology and Tissue Engineering

Artificial DNA synthesis

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Artificial DNA synthesis, sometimes known as DNA printing is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with pre-existing DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleo base pairs in addition to the two base pairs in nature, which could greatly expand the genetic code

Artificial DNA  synthesis  include :