Genomic Engineering

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Genomic Engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is a set of technologies used to change the gene cloning of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms.  Genes may be removed, or "knocked out", using a nuclease. Methods in genome engineering are: Insertion involves introducing a gene into a chromosome to obtain a new function. Inactivation, or “knock-out”, is to shed light on the function of a gene by observing the anomalies that occur as a result of its inactivation. Correction aims to remove and replace a defective gene sequence with a functional sequence. 

  • Multiplex Automated Genomic Engineering (MAGE)
  • Transfection by causing dsDNA breaks
  • Meganuclease-based Engineering
  • Zinc finger nuclease-based Engineering
  • TALEN
  • CRISPRs
  • Homologous recombination
  • rAAV-stimulated homologous recombination

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