Synthetic Biology and Genetic Engineering

Georgiy Yu. Shevelev has his expertise within the synthesis and investigation of physical and chemical properties of Nucleic Acids and their analogs and derivatives. One of other areas of his interests are: determination of thermodynamic parameters of DNA complexes formation. Investigation of spatial (2D and 3D) organization of DNA and DNA-protein complexes using physical methods. Visualization of biological objects using Atomic Force Microscopy (cells, viruses, proteins, nucleic acids, nanotubes etc.). Single molecule force spectroscopy using Atomic Force Microscopy. Organic synthesis of phosphoroamidites, non-nucleotide insertions. Automated oligonucleotide synthesis of native and modified Nucleic Acids. Labeling of Nucleic Acids and proteins with fluorophores and spin labels for Fluorescence and EPR spectroscopy. Aptamer technology (CE-SELEX, Non-Selex). Producing and purification of recombinant proteins. Basics of Molecular Dynamic simulations and visualization (Hyperchem 8.0, Chimera).