Malathi Raman
Takara Bio Europe, France
Title: Next-gen cloning and purification technologies to rapidly generate synthetic genes and proteins
Biography
Biography: Malathi Raman
Abstract
There is a constant need for faster and more efficient cloning and protein purification methods at all scales within the Synthetic Biology field. Scientists can accelerate the generation of synthetic gene expression constructs using our innovative Next-Gen In-Fusion® HD Cloning Plus technology which is fast (15 mins), highly efficient (>95% cloning efficiency), sequence independent (any insert can be cloned into any vector at any locus), seamless (no extra bp), directional, and HTP ready. After generation of the required expression construct and downstream expression of the target protein, Takara offers Next-Gen Capturem™ technology to allow fast (5-15 mins) and easy resin free purification of high quality and concentrated His-tagged proteins or native untagged antibodies. Our revolutionary Capturem technology, available in miniprep, maxiprep and 96-well plate formats, consists of spin columns or plates containing high-capacity nylon membranes immobilized with either Ni2+ or Protein A, thus allowing His-tagged protein or antibody purification directly from even complex matrices, such as cell supernatants or serum, within minutes. This talk will review several applications of our technologies including HTP antibody cloning, simplified purification of membrane and secreted proteins, fast hybridoma screening, and rapid immunoprecipitation (IP)/co-immunoprecipitation (Co-IP). We will also introduce our new Capturem Trypsin technology that enables 1 min on-column tryptic digests.
