Sharon Mendel Williams
Coventry University, UK
Title: Exploring unique compounds from lignin degradation using MnSOD and DyP-type peroxidase enzymes
Biography
Biography: Sharon Mendel Williams
Abstract
Lignin is an organic polymer found in the cell walls of plants. Lignin can be used to create biofuels, or as an organic hydrocarbon source for a large variety of chemicals and polymers. However, lignin is very robust and current industrial processes for using it are inefficient. Therefore, a useable biological process for degrading lignin would be of great benefit. Understanding the pathway of lignin degradation, and the byproducts, is essential in order to be able to exploit the use of micro-organisms. Furthermore, we can characterize novel bio-products obtained by enzymatic oxidation of lignin, which could have very interesting applications for industrial biotechnology. In the current project DyP-type peroxidases from Gram-negative Pseudomonas fluorescens Pf-5 and recombinant Sphingobacterium MnSOD1 and MnSOD2 cloned into E. coli and were investigated. These are bacterial enzymes that are already known to degrade lignin. Different genetic mutations were introduced, and the resulting enzymes were characterized by using them with different lignin substrates. The reaction compounds were analyzed by reverse phase HPLC/GC-MS. The goal is to improve the effectiveness of the enzymes, increase the production of the enzymes and degrade the lignin into different and more useable compounds. Any of these goals would be of valuable scientific and commercial benefit.
