JEEVITHAN ELANGO,
Shanghai Ocean University, China
Title: Characterization of Type-II Fish collagen extracted from Shark cartilage and its potential effect on Rheumatoid arthritis
Biography
Biography: JEEVITHAN ELANGO,
Abstract
Type-II pepsin soluble collagens (PSC) were isolated from cartilage of shark; and examined for their biochemical and antioxidant properties. The purified collagen was composed of an identical (α1)3 chains. The biochemical properties such as viscosity, solubility, amino acid composition, glycoprotein content, SEM, FTIR, CD and DSC spectra; and antioxidant properties substantiated that PSC could be used as a suitable biomaterial for pharmaceutical and biomedical industries as an alternative source of mammalian collagen. From the gut-sac (in-vitro intestinal absorption) experiments, we identified hydrolysates within a MW range from 70 kD to 8 kD on the serosal side of the intestine after the application of collagen hydrolysates and a significant amount of collagen hydrolysate is absorbed in the molecular form of 35-40 kDa. In continuation, type-II collagenous polypeptide (37 kDa) was successfully separated from the hydrolysates and studied for their physico-functional and antioxidant properties. Further, we target to confirm the effects of oral tolerance of PSC on inflammatory and immune responses to the ankle joint of rheumatoid-arthritis (RA) rats induced by Complete Freund’s Adjuvant. The immunological indexes such as delayed type hypersensitivity, IL-10 and T cell apoptosis showed that PSC with concentration of 10 μg/L could have significant effect in the model. The result of histological staining indicated that the recovery of the articular membranes of ankle joint in PSC group was greatly enhanced. In conclusion, the present results suggest that appropriate dose of PSC can not only ameliorate symptoms but also modify the disease process of RA.