Seyed Abdolreza Mortazavi Tabatabaei
Tehran University of Medical Sciences, Iran
Title: Adipocyte derived mesenchymal stem cell supports keratinocyte growth in a modified collagen-hyalorunic acid matrix
Biography
Biography: Seyed Abdolreza Mortazavi Tabatabaei
Abstract
Skin loss can occur due to many reasons including burns, diabetic wounds, venous ulcers, trauma, genetic disorders and etc... . Although much progress has been made towards the development of skin engineering but these are not completely efficient and require to improvement. It is known that many types of cells including fibroblasts and bone marrow stem cells improve performance of composite skin substitutes. Secretion of collagen matrix and epidermal differentiation takes place after mesenchymal epithelial communication. Development of the better transplantable dermal layers that support keratinocyte proliferation is very important for therapeutic option. Here we worked on an autologous construct as a successful skin substitute by culture of human Adipocyte Derived Mesenchymal stem cells (hADMSC) and human keratinocytes on the Hyalorunic acid-Collagen matrix. Bi-layer skin culture was generated by using biodegradable Hyalorunic acid-Collagen matrix. To analyze the effects of the hADMSC on the epithelial regeneration, keratinocytes were seeded onto the hADMSC-populated matrix and cultured at 37°C, in a 5% CO2 and 20% O2 humidified atmosphere. First, 106 hADMSC were seeded onto the matrix and cultured for 3 days in the basal medium. Then, keratinocytes were overlaid on the hADMSC and cultured for other 7 days in complete keratinocyte medium. We have evaluated the growth behavior of hADMSC and keratinocytes as a co-culture on the Col-HA layer. The obtained results revealed that the designed co-culture has a high potential for human keratinocyte proliferation. Keratinocytes without ADMSC-support formed only an irregular layer. This suggests that mesenchymal intercellular communications are necessary for proliferation and stratification of human keratinocytes. Keratinocytes cultured with hADMSC expressed Ck10 and Ck14 in supra-basal layer. However ADMSC is not a part of normal skin but it could promote the epidermal regeneration as describes previously for the bone marrow stem cell and other progenitor cells. Our data propose ADMSC as an efficient and safe source of feeder cells for the generation of keratinocyte autografts without immunological complications.