Next Generation Sequencing

Massive parallel sequencing or massively parallel sequencing is any of several high-throughput approaches to DNA sequencing using the concept of massively parallel processing; it is also called next-generation sequencing (NGS) or second-generation sequencing. Through NGS an entire human genome can be sequenced within a single day. In contrast, the previous Sanger sequencing technology, used to decipher the human genome, required over a decade to deliver the final draft. Although in genome research NGS has mostly superseded conventional Sanger sequencing, it has not yet translated into routine clinical practice. The aim of this article is to review the potential applications of NGS in paediatrics .Innovative NGS sample preparation and data analysis options enable a broad range of applications.
 
There are several methods to achieve Next Generation Sequencing:

  • Advanced methods and de novo sequencing
  • High-throughput methods
  • Maxam-Gilbert sequencing
  • Chain-termination methods

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